Stimultaneous Determination of 11 Flavonoids in Citrus by Ultra Performance Liquid Chromatography

Abstract

Abstract:

A rapid method for qualitative and quantitative analysis of 11 flavonoids in citrus was developed using ultra performance liquid chromatography (UPLC) coupled with photodiode array detector. Under the condition of methanol-.acetic acid water solution as mobile phase and gradient elution, 8 flavanones and 3 polymethacrylate flavones were separated to baseline within 11 min. The detection wavelengthes were 283nm and 330nm. The limits of detection (LODs) were from 0.005 to 0.02mg/kg (S/N=3) and good linearity was observed with the correlation coefficient (r2)≥0.9991.The influencing factors sample preparation were optimized by using Orthogonal experimental design and the recoveries of compounds analyzed from peel and juice samples were in the range of 94.6–101.2% with RSD between 1.1 and 3.7%, and in the range of 94.5–101.8% with RSD between 0.9 and 3.9%, respectively.Compared with the existing, this method was more effective, environmentally friendly and reproducible.

CLC Number:

S666

References

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