Abstract:

A method for the determination of organic and inorganic selenium in duck eggs by Hydride Generation-Atomic Fluorescence Spectrometry (HG-AFS)had been developed. A sample was extracted with the buffer solution of Tris (hydroxymethyl) aminomethane-hydrochloric acid (Tris-HCl) in PH=8.6，deposited with Ammonium sulphate. The total selenium and inorganic selenium content were detected with HG-AFS. The content difference between total selenium and the inorganic selenium was organic selenium content. The detection limit for selenium was 0.003mg/kg under the optimum condition. The linear range was 2.0-50μg/L.The relative standard derivation was less than3.21%. The recoveries was 82.8-91.0%. The inorganic selenium and organic selenium content were detected respectively from the selenium enriched duck eggs by this method. This method met the determination of organic selenium in duck eggs．