Extraction and Determination of Total Alkaloids from Fritillariae Bulbs by Response Surface Methodology

Abstract

Abstract: In order to achieve the optimal processing conditions of alkaloids from Fritillariae bulbs. Fritillaria cirrhosa was firstly investigated. Response surface design were used to explore the extractive rate of the alkaloids through changing solvent ratio (CHCl3:CH3OH), liquid volume and the extraction time. The content was determined by colorimetry. Results indicated that the optimal extractive conditions were solvent ratio (CHCl3:CH3OH) of 4.2:1, liquid volume of 30, and extaction of 4 h. The maximum total alkaloids of 5.379 mg/g was from Fritillaria hupehensis under the optimal extractive conditions. This method is reliable, easy to operate. The optimized extractive conditions of should be the best for the extraction of the Fritillaria bulbs.

CLC Number:

TS201.1

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