Abstract: This paper in order to obtain high purity lutein, with corn protein powder as raw materials,to study the extraction process conditions for lutein by supercritical CO2,taking the HPLC as the determination method. The technology parameter of lutein extraction was studied by response surface analytic.Based on the single factor experiment,selecting extraction time,temperature and pressure three main factors,with the purity of lutein as response value to optimize the extraction process parameters and gain the optimum extraction process conditions:extraction time is 2.3h,temperature is 42℃,pressure is 30 MPa,φ(ethanol)=90% as the entrainer and the additive amount is 5%,by which the lutein purity is 46.8% at these conditions and the verification is 46.5%.