The research of the extracting, purification and the hypoglycemic effect of Lycium chinense. leaf polycose

Abstract

Abstract: Response surface methodology (RSM) was applied to optimize the best extracting method of Lycium chinense. leaf. Model equation was proposed with regard to the effect of the ratio of Lycium chinense. leaf powder to water , temperature and time. The optimum conditions for the effect of the ratio of Lycium chinense. leaf powder to water , temperature and time obtained using RSM with regard to the content of polycose were 1:47, 72.9℃, and 2 h 12min respectively. The crude polycose was then purified by D 101 macroporous resin and DEAE-52 column. Phenol-sulfuric acid method was chosen to test the purity of sample which was up to 92.5%. The anti-diabetic activity of the polycose was assessed using enzymatic inhibitory assays against the α-glycosidase. The percentage of the inhibition of U/mL α-glycosidase is 93.7% however the percentage of the inhibition of control acarbose was only 21.7%.

CLC Number:

References

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