Abstract:

A method for the determination of three monoglycerate, mono-glycerate glycerate monocaprylin, glyceryl
monodecanoate and glyceryl monolaurate, in sausage by GC-MS was established. Samples were extracted with
dichloromethane-n-hexane (1:1), and the extract was concentrated, purified by gel permeation chromatography (GPC), and
detected and confirmed by GC-MS. The quantitative analysis was conducted by using an external standard method. The limit
of quantification was 50 mg/kg. Satisfactory linearity was observed with a correlation coefficient of no less than 0.999 6 in
the concentration range of 20?1 000 μg/mL. Spiked recoveries were in the range of 81.3%?105% with RSD of 3.64%?8.56%
(n = 8). The method is rapid, convenient, accurate and precise, which can be applied to determine monoglycerate in sausage.

Key words: gas chromatography-mass spectrometry (GC-MS), sausage, monoglycerate