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Purification of Polyphenols from Inonotus obliquus by Macroporous Resin and Its Component Analysis

DONG Qi1, GAO Shan1, CAO Longkui1,2,*   

  1. 1. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China;
    2. National Coarse Cereals Engineering Research Center, Daqing 163319, China
  • Online:2015-11-25 Published:2015-12-03
  • Contact: CAO Longkui

Abstract:

The crude polyphenol extract from Inonotus obliquus was purified by macroporous resin adsorption and the
purified compounds were identified by high performance liquid chromatography-electrosprary ionization-mass spectrometry
(HPLC-ESI-MS). The results showed that D101 resin was suitable for the purification of polyphenols from Inonotus obliquus
due to the maximum static adsorption capacity and rate, and excellent desorption performance. The optimal conditions for
the purification process were determined as follows: the sample containing 1.0 mg/mL of polyphenols was loaded at a flow
rate of 1.0 mL/min followed by elution with 70% aqueous ethanol at a flow rate of 1.0 mL/min. Under these conditions, the
purity of polyphenols was increased from 16.52% to 49.77%. HPLC-ESI-MS analysis showed that 9 individual polyphenols
were purified from Inonotus obliquus including leucoanthocyanidins, astilbin, curculigoside, quercetin-3-O-glycoside,
catechin-7-xyloside, cucurbitacinⅡb, tangeretin, c-atechin, and rutin.

Key words: macroporous resin, purification, Inonotus obliquus polyphenol, component analysis

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