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Determination of Ascorbic Acid Based on its Inhibition on Nano-Ceria Chemiluminescence

SHI Wenbing, HE Wei*, WANG Bangjiang   

  1. Chongqing Key Laboratory of Inorganic Special Functional Materials, College of Chemistry and Chemical Engineering,
    Yangtze Normal University, Chongqing 408100, China
  • Online:2015-12-25 Published:2015-12-24
  • Contact: HE Wei

Abstract:

A new flow injection chemiluminescence (CL) method based on the inhibition of ascorbic acid on nano-ceria-
H2O2-luminol CL system has been developed for the determination of ascorbic acid (AA). The effects of concentrations of
H2O2, luminol and nano-ceria, and pH on CL intensity were investigated respectively. Under the optimum conditions, the
linear range of the method was 0.001–1.0 mg/mL (r = 0.983 1), and the detection limit (3σ) was 0.2 ng/mL. The relative
standard deviation (RSD) for 11 repetitive determinations of 1.0 mg/mL AA was 2.9%. The method is simple, sensitive and
fast and has been successfully applied to determine ascorbic acid in fruits, juices and vegetables.

Key words: nano-ceria, chemiluminescence, ascorbic acid

CLC Number: