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Analysis of Volatile Compounds in Rose Hydrolat by Headspace Solid-Phase Micro Extraction and Gas Chromatography-Olfactometry-Mass Spectrometry

MIAO Xiaoxiao, LI Meiping, LI Ping, FENG Xiaoxiao, REN Hongbin, ZHANG Shengwan   

  1. College of Life Science, Shanxi University, Taiyuan 030006, China
  • Online:2016-06-25 Published:2016-06-29
  • Contact: ZHANG Shengwan E-mail:zswan@sxu.edu.cn

Abstract:

The volatile compounds in rose hydrolat were analyzed by headspace solid-phase micro extraction (HS-SPME)and gas chromatography-olfactometry-mass spectrometry (GC-O-MS). Extraction conditions and GC-MS conditions weresystematically studied and the contributions of the main components to the overall aroma of rose hydrolat were evaluatedby odor activity value (OAV) and aroma intensity (AI) value. The optimal extraction efficiency was achieved under thefollowing conditions on Rt-Wax column: 50/30 μm divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS)SPME fiber, 8.0 mL of sample in a 20 mL vial; temperature, 75 ℃; extraction time, 40 min; equilibrium time, 20 min;and desorption time, 4 min. The results showed that a total of 92 compounds were detected, 73 compounds of which werestructurally identified, accounting for 98.77% of the total volatile substances. Evaluation based on OAV and AI valuesindicated that eugenol, rose oxide, geraniol, linalool, eugenol methyl ether, and benzene ethanol were the main aromacomponents. The results of this study can provide a theoretical basis for the study on aroma characteristics of rose hydrolatand the development and application of rose hydrolat.

Key words: headspace solid-phase micro extraction, gas chromatography-olfactometry-mass spectrometry (GC-O-MS), rose hydrolat, volatile compounds

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