FOOD SCIENCE ›› 2021, Vol. 42 ›› Issue (15): 136-142.doi: 10.7506/spkx1002-6630-20200918-237

• Nutrition & Hygiene • Previous Articles     Next Articles

Curcumin Attenuated Hypoxia/Reoxygenation-Induced Injury in LLC-PK1 Cells via Na/K-ATPase/Src Signaling Pathway

ZHAI Bingzhong, ZHANG Lijing, LIU Zhen, CHEN Jianguo, HU Zhihang, MEI Song, HU Wenli, LOU Minhan, WANG Yin, QU Xuefeng   

  1. (School of Food Science and Engineering, Hangzhou Medical College, Hangzhou 310013, China)
  • Online:2021-08-15 Published:2021-08-27

Abstract: Objective: This study aimed to elucidate the role of curcumin as an incomplete promoter of Na/K-ATPase (NKA) in protecting cells from hypoxia/reoxygenation (H/R)-induced injury by inducing the activation of the NKA/steroid receptor coactivator (Src) receptor complex. Methods: The effects of curcumin on the activity and conformation of NKA were determined by NKA screening test. An H/R model was used to simulate oxidative damage in cells. After LLC-PK1 cells were pre-protected with curcumin for 1 h and then treated with hypoxia for 1 h followed by reoxygenation for 3 h, the activity of extracellular lactate dehydrogenase (LDH), cell viability, and the expression levels of Src, phosphorylated steroid receptor coactivator (p-Src), extracellular signal-regulated protein kinases (Erk) and phosphorylated extracellular signal-regulated protein kinases (p-Erk) were examined. Results: Curcumin could inhibit the activity of NKA, change its conformation and activate the NKA/Src signaling pathway through increasing the phosphorylation levels of Src and Erk1/2. However, the co-existence of curcumin and ouabain could increase the activity of NKA and inhibit the over-activation of the NKA/Src signaling pathway. Curcumin attenuated oxidative stress-induced injury in LLC-PK1 cells, decreased the level of LDH activity, increased cell viability, and effectively lowered the expression levels of p-Src and p-Erk. Conclusion: Curcumin has a bi-directional regulatory effect on the NKA/Src signaling pathway. It could protect cells from H/R by inducing the activation of the NKA/Src receptor complex.

Key words: curcumin; Na/K-ATPase; steroid receptor coactivator kinase; extracellular signal-regulated protein kinases; oxidative damage

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