FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (4): 53-58.doi: 10.7506/spkx1002-6300-201004012

• Processing Technology • Previous Articles     Next Articles

Camellia chrysantha (Hu) Tuyama Leaves: Optimization of Ultrasonic-assisted Extraction and Methodological Evaluation of Phenol-Sulfuric Acid Determination of Polysaccharide

XU Zi-jing,LIAO Min-fu,CHEN Hai-yan,JIANG Yan,LIN Cui-wu*   

  1. School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China
  • Received:2009-02-09 Online:2010-02-15 Published:2010-12-29
  • Contact: XU Zi-jing E-mail:xuzj227@163.com

Abstract:

Optimal ultrasonic-assisted extraction of polysaccharide from Camellia chrysantha (Hu) Tuyama leaves (PCCT) was investigated by single factor method and central composite design coupled with response surface methodology. In addition, a methodological evaluation was conducted for phenol-sulfuric acid determination of polysaccharide. Results showed that the optimal PCCT extraction conditions were as follows: 87 ℃ and water/material ratio 46:1 for a triplicate extraction duration of 54 min each time. Under such conditions, PCCT yield was up to 4.39%. Methodological evaluation demonstrated high precision, good repeatability and less error of developed phenol-sulfuric acid method for polysaccharide determination. Fresh Camellia chrysantha (Hu) Tuyama leaves was determined to contain 66.8 mg/g dry weight of polysaccharide by the method.

Key words: Camellia chrysantha (Hu) Tuyama leaves, polysaccharide (PCCT), extraction, response surface analysis (RSA), content determination

CLC Number: