Abstract:

In order to make more effective use of fish waste materials, acid-soluble and pepsin-soluble collagens (ASC and PSC) were extracted from swim bladders of grass carp and properties of ASC and PSC were investigated. The yields of ASC and PSC were 4.7% and 11.1%, respectively, on the basis of dry weight. UV spectra of ASC and PSC from grass carp exhibited the maximum absorption peak at 230 nm. Amino acid profiles of ASC and PSC revealed that contents of hydroxyproline and proline were 16.87% and 17.55%, respectively. SDS-PAGE patterns indicated that both ASC and PSC were composed of at least two different α-chains (α1 and α2), which was similar to porcine type I collagen. The denaturation temperatures (Td) of ASC and PSC were 33.0 ℃ and 34.8 ℃, respectively, about 3 － 4 ℃ lower than that of porcine skin collagen (37 ℃). These results suggested that collagens from swim bladders of grass carp had potential as an alternative or a supplementary source of collagen from land vertebrates being used in different fields for industrial purposes.

Key words: grass carp, swim bladder, collagen, characterization