Immobilization of Recombinant Chitinase with Two Functional Magnetic Carriers

doi:10.7506/spkx1002-6300-201003039

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (3): 173-176.doi: 10.7506/spkx1002-6300-201003039

• Bioengineering • Previous Articles Next Articles

Immobilization of Recombinant Chitinase with Two Functional Magnetic Carriers

FENG Li1,2 YAN Rui-xiang2 FENG Chun-yu2 LIN Yan1,2 LI Xi-hong1,*

1. College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China
2. National Engineering Technology Research Center for Preservation of Agricultural Products, Tianjin Key Laboratory of Postharvest
Physiology and Storage of Agricultural Products, Tianjin 300384, China

Received:2009-03-20 Revised:2009-09-15 Online:2010-02-01 Published:2010-12-29
Contact: LI Xi-hong E-mail:fengduhappy@yahoo.com.cn

Abstract

Abstract:

Two kinds of functional magnetic carriers (A-type and P-type) were prepared and used for the immobilization of recombinant chitinase. A-type carrier was prepared by 3-aminopropyltriethoxysilane (APTES) and Fe3O4 magnetic microsphere. P-type carrier was prepared by co-polymerization of glycidyl methacrylate (GMA) on the surface of Fe3O4 magnetic microsphere in the presence of polyethylene glycol. Results indicated that P-type carrier had higher protein loading capacity and purification fold compared with A-type carrier, which were 1689.74 mg/g and 6.8013, respectively. The activity of immobilized recombinant chitinase onto P-type carrier could maintain 69.66% at 4 ℃ for 10 days. The activity of immobilized recombinant chitinase onto P-type carrier remained more than 44 % of original activity after being repeatedly used 7 times.

Key words: chitinase, functional magnetic carrier, immobilization

CLC Number:

Q946.5

FENG Li1,2 YAN Rui-xiang2 FENG Chun-yu2 LIN Yan1,2 LI Xi-hong1,*. Immobilization of Recombinant Chitinase with Two Functional Magnetic Carriers[J]. FOOD SCIENCE, 2010, 31(3): 173-176 https://doi.org/10.7506/spkx1002-6300-201003039

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Immobilization of Recombinant Chitinase with Two Functional Magnetic Carriers

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