FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (22): 314-317.doi: 10.7506/spkx1002-6300-200922074

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Determination of Total Flavonoids in Larch by Spectrophotometry

WANG Yu,*   

  1. College of Chemistry and Chemical Engineering, Harbin Normal University, Harbin 150025, China
  • Received:2008-12-04 Online:2009-11-15 Published:2010-12-29
  • Contact: WANG Sui E-mail:wangsuiyzl@126.com

Abstract:

The determination accuracy of flavonoids in larch using three kinds of spectrophotometry methods was compared on the basis of determination of dihydroquercetin in larch using HPLC. Results indicated that direct determination of flavoniods in larch exhibited more accuracy than Al(NO3)3 and AlCl3 colorimetry. The linear equation was Y = 53.2714X+0.0153 (r= 0.9999). The excellent linear was shown in the range of 0.004-0.024 mg/ml. The relative standard deviation of this determination method was 1.36% and average recovery rate was 99.9%.

Key words: spectrophotometry, larch, total flavonoids

CLC Number: