Real-time PCR Assay of Trace Buckwheat (Fagopyrum spp.) in Foods

doi:10.7506/spkx1002-6300-200920068

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (20): 312-315.doi: 10.7506/spkx1002-6300-200920068

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Real-time PCR Assay of Trace Buckwheat (Fagopyrum spp.) in Foods

SHI Yan-yu1，LIU Jin-hua2，PANG Xiao-yang1，ZHANG Xiao-hui1，REN Chang-fei1

1. Jilin Product Quality Supervision Inspection, Changchun 130022, China；
2. Jilin Entry-Exit Inspection and Quarant Bureau, Changchun 130062, China

Received:2008-10-20 Revised:2009-07-30 Online:2009-10-15 Published:2010-12-29
Contact: SHI Yan-yu E-mail:shiyanyu219@yahoo.com.cn

Abstract

Abstract:

A real-time PCR method for the detection of trace buckwheat in foods was established using forward and reverse primers and probe corresponding to the internal transcribed spacer region (ITS) and the 5.8S rRNA gene. The method was buckwheat-specific and compatible with both cultivated and wild buckwheat of the Fagopyrum spp. Its sensitivity was sufficient to detect 0.1 μg/kg of buckwheat DNA spiked in wheat DNA.

Key words: buckwheat allergy, internal transcribed spacer (ITS), real-time PCR, detection

CLC Number:

TS207

SHI Yan-yu1，LIU Jin-hua2，PANG Xiao-yang1，ZHANG Xiao-hui1，REN Chang-fei1. Real-time PCR Assay of Trace Buckwheat (Fagopyrum spp.) in Foods[J]. FOOD SCIENCE, 2009, 30(20): 312-315.

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Real-time PCR Assay of Trace Buckwheat (Fagopyrum spp.) in Foods

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