Abstract:

Objective: To develop a technique for synchronized and efficient extraction of flavonoids and alkaloids [the main component in alkaloids from mulberry leaves was 1-deoxynojirimycin (DNJ)] from mulberry leaves. Methods: The optimal pH value of acidic ethanol solution for extraction was confirmed by single factor test, and then other 4 extraction parameters were optimized by orthogonal design based on single factor test. Results: The best synchronized extraction effect of flavonoids and alkaloids was achieved under the following conditions: with ratio of 60% acidic ethanol solution at pH 2 to dry mulberry leaves powder of 10:1, duplicate extraction at 80 ℃ for 2 h. Conclusion: Orthogonal design is feasible to optimize the synchronized extraction of flavonoids and alkaloids from mulberry leaves.

Key words: mulberry leaves, flavonoids, alkaloids, 1-deoxynojirimycin (DNJ), extraction process