Immunoassay Determination of Tartrazine

doi:10.7506/spkx1002-6630-200912059

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (12): 258-261.doi: 10.7506/spkx1002-6630-200912059

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Immunoassay Determination of Tartrazine

REN Li-song1,2，LU Shi-ying1，ZHOU Yu1，Guo De-jun1，LI Yan-song1，MENG Xian-mei1，LIU Zeng-shan*

(1. Institute of Zoonosis, Jilin University, Changchun 130062, China；
2. Xinjiang Military Center for Disease Control and Prevention, Urumqi 830011, China)

Received:2008-05-06 Revised:2008-12-22 Online:2009-06-15 Published:2010-12-29
Contact: LIU Zeng-shan* E-mail:zsliu1959@sina.com

Abstract

Abstract:

Tartrazine was coupled separately with bovine serum albumin (BSA) and ovalbumin (OVA) by 1-ethyl-3-(3- dimethylaminopropyl) carbodiimide (EDC) to prepare complete antigens. Then hybridoma cell lines secreting monoclonal antibody (McAb) against tartarzine were obtained by immunization of BALB/C mice with prepared BSA- tartarzine and then fusion between SP2/O myeloma cells and splenocytes from the immunized mice, and the McAb against tartarzine was prepared. Finally, an indirect competitive ELISA method was established for determination of tartrazine. The regression equation was y =－40.134x + 133.46, with a determination coefficient of 0.9692. The limit of detection was 0.36 μg /L. Compared to the widely used methods such as TLC and spectrophotometric assay, this new method is found to be time-saving, easy to perform, highly specific and sensitive. It was suitable for the determination of tartarzine-containing samples in large quantity.

Key words: tartarzine, McAb, ELISA, detection

CLC Number:

R155.5

REN Li-song1,2，LU Shi-ying1，ZHOU Yu1，Guo De-jun1，LI Yan-song1，MENG Xian-mei1，LIU Zeng-shan*. Immunoassay Determination of Tartrazine[J]. FOOD SCIENCE, 2009, 30(12): 258-261.

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Immunoassay Determination of Tartrazine

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