FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (10): 38-41.doi: 10.7506/spkx1002-6630-200910003

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Optimization of Extraction Technology of Polysaccharides from Gracilaria lemaneiformis at Room Temperature and Their Immunocompetence

GONG Chun-yu1,2，ZHANG Jin-song2，TANG Qing-jiu2，LIU Yan-fang2，YE Li-bin3，DU Xiu-ju3，PAN Ying-jie1,*   

  1. (1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 200090, China；
    2. Edible fungus Research Institue, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China；
    3. College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China)
  • Received:2008-08-21 Revised:2008-11-23 Online:2009-05-15 Published:2010-12-29
  • Contact: PAN Ying-jie1,* E-mail:yjpan@shou.edu.cn

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Abstract:

The extraction technology of crude polysaccharides from Gracilaria lemaneiformis (GCp) at room temperature was optimized by orthogonal test, and the inducing effects of GCp and different fractions of GCp obtained by different methods on proliferation of mouse lymphocyte were investigated. The results indicated that extraction times and extraction time were the significant factors affecting the yield of GCp. The maximum yield of 9.96% was obtained by extracting three times for 12 h at the solid to liquid ratio of 1:20. 32% of protein contained in crude polysaccharide could be removed by Sevag method (repeated 5 times). The inducing effect of GCp extracted at room temperature on proliferation of mouse lymphocyte was significantly higher than thoseot obtained at 100 ℃ and 121 ℃ at the same concentration. Five kinds of polysaccharides were obtained by ultrafiltration, freeze-thaw and Sevag method (GCpF1 and GCpF2 by ultra-filtration, GCp-1 and GCp-2 by freeze-thaw, and GCp- S by Sevag method). The immunocompetence assay revealed that all GCp and the five polysaccharide fractions were able to induce proliferation of mouse lymphocyte in a concentration-dependent manner, and GCp, GCpF1 and GCpF2 were more active than GCp-1, GCp-2 and GCp-S.

Key words: Gracilaria lemaneiformis, polysaccharides, orthogonal design, ultra-filtration, freeze-thaw, immunocompetence

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