Screening and Breeding of High-yield Transglutaminase (TGase)-producing Streptomyces hygroscopicus Strain Resistant to 2-deoxy-D-Glucose Repression

doi:10.7506/spkx1002-6630-200909001

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (9): 120-123.doi: 10.7506/spkx1002-6630-200909001

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Screening and Breeding of High-yield Transglutaminase (TGase)-producing Streptomyces hygroscopicus Strain Resistant to 2-deoxy-D-Glucose Repression

XU Xiao-juan LI Jiang-hua ZHANG Dong-xu CHEN Jian DU Guo-cheng*

Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University,
Wuxi 214122, China

Received:2008-08-18 Revised:2008-12-18 Online:2009-05-01 Published:2010-12-29
Contact: DU Guo-cheng E-mail:gcdu@jiangnan.edu.cn

Abstract

Abstract:

In the present study, through mutagenizing Streptomyces hygroscopicus CCTCC M203062 with UV and N-implantation, a mutant strain BB-2 with TGase activity of 7.19 U/ml was obtained. Then a new stain DG-1 resistant to 2-deoxy-D-glucose (2-DG) repression was obtained from the mutant strain BB-2 by repeated UV radiation, with good genetic stability and TGase activity of 9.21 U/ml, which was higher 118.5% than that of the original strain CCTCC M203062.

Key words: TGase, Streptomyces hygroscopicus, UV mutagenization, N+-implantation, 2-deoxy-D-glucose

CLC Number:

Q 933

XU Xiao-juan LI Jiang-hua ZHANG Dong-xu CHEN Jian DU Guo-cheng*. Screening and Breeding of High-yield Transglutaminase (TGase)-producing Streptomyces hygroscopicus Strain Resistant to 2-deoxy-D-Glucose Repression[J]. FOOD SCIENCE, 2009, 30(9): 120-123.

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Screening and Breeding of High-yield Transglutaminase (TGase)-producing Streptomyces hygroscopicus Strain Resistant to 2-deoxy-D-Glucose Repression

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