Abstract:

Based on single-factor test and orthogonal test, microwave-assisted extraction conditions of total flavonoid  from peanut hulls were optimized. The results showed that the optimal extraction conditions are as follows: taking 50% alcohol as extraction solvent, microwave power 700 W, radiation time 4 min and material to extraction solvent ratio 1:30 (g/ml). Under the above conditions, the yield and spike recovery of total flavonoid are 0.48 mg/g and 93.3%, respectively. Compared to ethanol reflux extraction, microwave-assisted extraction not only can reduce the extraction time from 4 h to 4 min but improve the yield by 2.4 times. Furthermore, the purification of total flavonoids from peanut hulls by macroporous adsorption resin was also investigated in the present study. Using the luteolin content as the index, the capabilities of static adsorption and desorption were examined. The results showed that the D101 resin is the best among chosen 7 resins for the purification, and both of contents of total flavonoid and luteolin are promoted by 4 to 5 times after purification.

Key words: peanut hulls, microwave, flavonoid, adsorption resin, luteolin