FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (7): 154-158.doi: 10.7506/spkx1002-6630-200907035

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Optimization of Protoplast Regeneration Medium Composition for Streptomyces gilvosporeus by Response Surface Methodology

LUO Jian-mei LI Jian-shu GUO Hao LIU Feng LIU Dan WANG Min   

  1. (Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and
    Technology, Tianjin 300457, China)
  • Received:2008-07-10 Revised:2008-11-13 Online:2009-04-01 Published:2010-12-29
  • Contact: LUO Jian-mei E-mail:luojianmei@tust.edu.cn

Abstract:

In order to promote the regeneration rate, three components of protoplast regeneration medium for Streptomyces gilvosporeus were optimized by means of single-factor design and response surface methodology. The results showed that the optimal concentrations of KH2PO4, L-proline and polyvinylpyrrolidone are 0.13, 5.48 and 0.18 g/L, respectively. Under the above conditions, the protoplast regeneration rate reaches 17.2%, which well accords with the model prediction value of 16.7%. It was indicated that response surface methodology is an effective way to optimize regeneration medium composition of protoplast.

Key words: Streptomyces gilvosporeus, protoplast, regeneration medium, regeneration rate, response surface methodology

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