Improved Flinders Technology Associates (FTA) Filter Paper-based DNA Extraction with Polymerase Chain eaction (PCR) for Rapid Detection of Listeria monocytogenes

doi:10.7506/spkx1002-6630-200906044

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (6): 196-198.doi: 10.7506/spkx1002-6630-200906044

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Improved Flinders Technology Associates (FTA) Filter Paper-based DNA Extraction with Polymerase Chain eaction (PCR) for Rapid Detection of Listeria monocytogenes

LI Hui，ZHANG Hui-yan，MA Xiao-yan，LIU Yi，HE Yi，ZHANG Wei*

(College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China)

Received:2008-05-20 Revised:2008-08-28 Online:2009-03-15 Published:2010-12-29
Contact: ZHANG Wei* E-mail:zhangwei631126@yahoo.com.cn

Abstract

Abstract:

Objective: To set up a detection approach of Listeria monocytogenes based on PCR. Methods: A pair of primers ere designed with hlyA gene of Listeria monocytogenes as target sequence for its detection by improved FTA-PCR method. esults: A 136-bp DNA fragment was amplified from the primers. The sensitivity of the improved FTA-PCR method was up o 1.1 × 102 CFU/ml. Conclusion: This method is rapid, sensitive and accurate, with high specificity, which can shorten the etection time from 7 to 14 days to 1 to 1.5 days.

Key words: Listeiamonocytogenes, TA-PCR, detection

CLC Number:

TS207.3

LI Hui，ZHANG Hui-yan，MA Xiao-yan，LIU Yi，HE Yi，ZHANG Wei*. Improved Flinders Technology Associates (FTA) Filter Paper-based DNA Extraction with Polymerase Chain eaction (PCR) for Rapid Detection of Listeria monocytogenes[J]. FOOD SCIENCE, 2009, 30(6): 196-198.

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Improved Flinders Technology Associates (FTA) Filter Paper-based DNA Extraction with Polymerase Chain eaction (PCR) for Rapid Detection of Listeria monocytogenes

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