Preliminary Study on Bacteriophage Infection of Pseudomonas fluorescens A46 and Corresponding Control

doi:10.7506/spkx1002-6630-200905046

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (5): 197-200.doi: 10.7506/spkx1002-6630-200905046

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Preliminary Study on Bacteriophage Infection of Pseudomonas fluorescens A46 and Corresponding Control

ZHANG Zhi-ping1，LI Xiao-ming1，SUN Wen-jing1,2，WANG Duo1，LIU Jing-ze1,*

(1. College of Life Sciences, Hebei Normal University, Shijiazhuang 050016, China；
2. College of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China)

Received:2008-04-28 Revised:2008-09-02 Online:2009-03-01 Published:2010-12-29
Contact: LIU Jing-ze1,* E-mail:liujingze@mail.hebtu.edu.cn

Abstract

Abstract:

The changes of cell morphology of Pseudomonas fluorescens A46, a strain producing 2-keto-D-gluconic acid, were observed by transmission electron microscope after infected by bacteriophage KS461-2. The mutation frequency of P. fluorescens A46 resisting the bacteriophage was determined, the multiplication of the bacteriophage was studied at different pH values, and the control effects of four medicaments on the bacteriophage infection were investigated. The current results showed that the morphology significantly changes following with infection time extending, the mutation frequency of P. fluorescens is about 2.5×10-6, the bacteriophage easily adheres to the host bacteria and proliferates in the pH range of 7.5 to 8.0, and ammonium oxalate can remarkably restrain the multiplication of the bacteriophage and does not affect the growth of P. fluorescens A46, especially the addition of 0.7% is the best.

Key words: Pseudomonas fluorescens, 2-keto-D-gluconic acid, bacteriophage, infection, cell morphology

CLC Number:

TS 201.3

ZHANG Zhi-ping1，LI Xiao-ming1，SUN Wen-jing1,2，WANG Duo1，LIU Jing-ze1,*. Preliminary Study on Bacteriophage Infection of Pseudomonas fluorescens A46 and Corresponding Control[J]. FOOD SCIENCE, 2009, 30(5): 197-200.

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Preliminary Study on Bacteriophage Infection of Pseudomonas fluorescens A46 and Corresponding Control

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