FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (4): 76-80.doi: 10.7506/spkx1002-6630-200904011

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Extraction of Type II Collagen from Chicken Sternal Cartilage Powder and Its Structural Analysis

LU Xue-qin,XU Shi-ying *,CAO Hui   

  1. (School of Food Science and Technology, Jiangnan University, Wuxi 214122, China)
  • Received:2008-01-30 Revised:2008-09-01 Online:2009-02-15 Published:2010-12-29
  • Contact: LU Xue-qin E-mail:lxq_lulutong@yahoo.com.cn

Abstract:

The material powder was prepared from chick sternal cartilage, hydrochloric carbamidine was used to remove non-collagen components, and then pepsin was added to extract type II collagen. The results of SDS-PAGE electrophoresis and RP-HPLC showed that the extracted type II collagen has high purity. Analysis results of amino acid components, UV absorbance spectrogram, infrared spectrum, circular dichroism (CD) spectrogram and differential scanning calorimeter (DSC) proved that type II collagen from cartilage powder remains its natural structure.

Key words: chicken sternal cartilage, type II collagen, structure

CLC Number: