Isolation and Purification of Usnic Acid from Usnea longissima by Preparative High-speed Counter-current Chromatography

doi:10.7506/spkx1002-6630-200903027

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (3): 127-129.doi: 10.7506/spkx1002-6630-200903027

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Isolation and Purification of Usnic Acid from Usnea longissima by Preparative High-speed Counter-current Chromatography

CHI Chun-yan1，SHI Bo1,*，CHENG Yong-qiang2，LIANG Ping1，LI Jing-mei3

(1. Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China；
2. College of Food and Nutritional Engineering, China Agricultural University, Beijing 100083, China；
3. Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

Received:2008-02-28 Revised:2008-05-05 Online:2009-02-01 Published:2010-12-29
Contact: SHI Bo1,*， E-mail:shibo@mail.caas.net.cn

Abstract

Abstract:

Preparative high-speed counter-current chromatography (HSCCC) was used for the isolation and purification of usnic acid from Usnea longissima. Crude extract was firstly obtained by reflux extraction with petroleum ether at 60 to 90 ℃ for 4 h from dried Usnea longissima. Then hexane-acetonitrile-ethyl acetate-water (8:7:5:0.8, V/V) was used as the two-phase solvent system for purification. Finally the collected components were analyzed using high-performance liquid chromatography (HPLC) and the structure of main component was identified using 1H-NMR and 13C-NMR. By this way, the 11.2 mg of usnic acid is obtained from 25 mg of the crude extract of dried Usnea longissima with the purity of 99%.

Key words: high-speed counter-current chromatography, Usnea longissima, usnic acid, isolation, purification

CLC Number:

TS202.3

CHI Chun-yan1，SHI Bo1,*，CHENG Yong-qiang2，LIANG Ping1，LI Jing-mei3. Isolation and Purification of Usnic Acid from Usnea longissima by Preparative High-speed Counter-current Chromatography[J]. FOOD SCIENCE, 2009, 30(3): 127-129.

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Isolation and Purification of Usnic Acid from Usnea longissima by Preparative High-speed Counter-current Chromatography

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