Abstract: Russula cyanoxantha was cultured using liquid fermentation medium. Mycelia of Russula cyanoxantha were subjected to extraction by petroleum ether (PE), ethyl acetate (EA) and ethanol, respectively. Antioxidant activities of different extraction fractions were evaluated by scavenging capacity on DPPH free radicals and inhibitory rate of lipid peroxidation. Results indicated that EA-soluble extract and ethanol-soluble extract had better antioxidant activities and the ethanol-soluble extract revealed the highest antioxidant capability. MTT assay indicated that PE, EA and ethanol extracts had obvious inhibition effects on the proliferation of H446 cancer cells, suggesting the anti-tumor activities of these extracts. The compositions of ethanol extract from mycelia of Russula cyanoxantha were analyzed. Three fractions were achieved by chromatographic separation as Fraction I, II and III, respectively. Fraction I was identified as 1-aza-bicyclo [4.2.0] oct-4-en-8-one by IR, FAB-MS, 1H NMR, 13C NMR, HSQC and HMBC methods.

Key words: Russula cyanoxantha, mycelia, ethanol extract, antioxidant activity, anti-tumor activity