Comparative Study on Deproteinization Methods for Polysaccharide from Mactra veneriformis

Abstract

Abstract: Objective: To screen the optimum method for deproteinizing crude polysaccharide from Mactra veneriformis and to determine the polysaccharide content and biological activity of purified product. Methods: The deproteinization of crude polysaccharide from Mactra veneriformis was comparatively studied using ion exchange, trichloroacetic acid (TAC) method, PEG flocculation and salt fractionation. Results: Ion exchange, trichloroacetic acid (TAC) method and salt fractionation had good deproteinizing effect on crude polysaccharide from Mactra veneriformis, and the polysaccharide contents in the resulting purified products were 85%, 90.96% and 83%, respectively. PEG flocculation was unsuitable for the removal of protein impurities from crude polysaccharide from Mactra veneriformis. Ion exchange and TAC method provided highly effective hypoglycemic products, and the product derived from TAC method displayed a significant difference compared to the model control (P ＜ 0.05). Conclusion: The screened methods are feasible in practice.

Key words: crude Mactra veneriformis polysaccharide, deproteinnization, activity

CLC Number:

R282.7

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Comparative Study on Deproteinization Methods for Polysaccharide from Mactra veneriformis

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