Selection of Pre-enrichment Methods for Yersinia enterocolitica

doi:10.7506/spkx1002-6630-201105036

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (5 ): 168-171.doi: 10.7506/spkx1002-6630-201105036

• Bioengineering • Previous Articles Next Articles

Selection of Pre-enrichment Methods for Yersinia enterocolitica

NIU Lei1，ZHU Chang-qing1,2，ZHOU Guang-hong1,*，XU Xing-lian1， LI Bin1，JIANG Yun

1. Nantional Center of Meat Quality and Safety Control, Nanjing Agricultual University, Nanjing 210095, China；
2. Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing 200001, China

Received:2010-05-17 Revised:2011-01-18 Online:2011-03-15 Published:2011-03-03
Contact: NIU Lei E-mail:2008108060@njau.edu.cn

Abstract

Abstract: Yersinia enterocolitica has been paid so much attention as a kind of foodborne pathogen. The pre-enrichment of Yersinia enterocolitica was conducted by using the cultivation in TSB, PSB and ITC media. The pre-enrichment effect was evaluated by real-time PCR. The results indicated that the cultivation in TSB medium was better than other two media for pure Yersinia enterocolitica system. The enrichment time was 24 h. In addition, an enhanced sensitivity and shorten enrichment time (8 h) of Yersinia enterocolitica were achieved in the mixed microbe system containing TSB and ITC. Therefore, PSB can be used in the detection of Yersinia Enterocolitica in samples.

Key words: Yersinia enterocolitica, pre-enrichment, real-time PCR, culture medium, foodborne pathogen

CLC Number:

TS251.1

NIU Lei1，ZHU Chang-qing1,2，ZHOU Guang-hong1,*，XU Xing-lian1， LI Bin1，JIANG Yun. Selection of Pre-enrichment Methods for Yersinia enterocolitica[J]. FOOD SCIENCE, 2011, 32(5 ): 168-171 https://doi.org/10.7506/spkx1002-6630-201105036

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Selection of Pre-enrichment Methods for Yersinia enterocolitica

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