Preparation of Hydrolyzed Collagen from Cyprinoid Scales

doi:10.7506/spkx1002-6630-200924008

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (24 ): 51-55.doi: 10.7506/spkx1002-6630-200924008

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Preparation of Hydrolyzed Collagen from Cyprinoid Scales

HAN Bing1，SHI Yan-guo1，ZHANG Gen-sheng1，YANG Hui1，LI Yan-hong2

1. College of Food Engineering, Harbin University of Commerce, Harbin 150076, China；
2. Department of Food Engineering, Shandong Business Institute, Yantai 264670, Ch

Received:2009-08-16 Online:2009-12-15 Published:2010-12-29
Contact: HAN Bing1， E-mail:hb790118@163.com

Abstract

Abstract:

Decalcified cyprinoid scales were used as materials to prepare hydrolyzed collagen through alcalase hydrolysis. Pretreatment temperature, substrate concentration, hydrolysis temperature, enzyme concentration and hydrolysis time were optimized through evaluating extraction rate of collagen and content of hydroxyproline by single-factor and orthogonal experiments. Results indicated that optimal hydrolysis conditions were 60 ℃ of hydrolysis temperature, 12% substrate amount, 0.06 AU/g of enzyme, and 3 h of hydrolysis time. The extraction rate of collagen from cyprinoid scales was 96.24% under this optimal condition.

Key words: cyprinoid, scale, hydrolyzed collagen, hydrolysis

CLC Number:

TS254.9

HAN Bing1，SHI Yan-guo1，ZHANG Gen-sheng1，YANG Hui1，LI Yan-hong2. Preparation of Hydrolyzed Collagen from Cyprinoid Scales[J]. FOOD SCIENCE, 2009, 30(24 ): 51-55 https://doi.org/10.7506/spkx1002-6630-200924008

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Preparation of Hydrolyzed Collagen from Cyprinoid Scales

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