Abstract:

An easy and low-cost method for determining inosine monophosphate (IMP) in chicken by capillary zone electrophoresis (CZE) was established. The capillary zone electrophoresis was conducted in the buffer of 20 mmol/L borate (pH 9.3) at the voltage of 30 kV and detection wavelength of 254 nm. Results indicated that an excellent linear relationship between IMP concentration and peak area was observed (r = 0.9983). The relative standard deviations for migration time and peak area were less than 0.27% and 2.74%, respectively. This capillary electrophoresis method provided simple, rapid, convenient and high reproducible determination, which was suitable for the determination of IMP in chicken.

Key words: capillary zone electrophoresis, chicken, IMP