Expression of Core-Streptavidin Gene in Prokaryotic Host

doi:10.7506/spkx1002-6300-200923064

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (23 ): 286-289.doi: 10.7506/spkx1002-6300-200923064

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Expression of Core-Streptavidin Gene in Prokaryotic Host

PENG Fu-zhong1，CHEN Xue-lan1,*，XU Feng2，XIONG Yong-hua2

1. College of Life Science, Jiangxi Normal University, Nanchang 330022, China；2. State Key Laboratory of Food Science and
Technology, Nanchang University, Nanchang 330047, China

Received:2009-08-24 Online:2009-12-01 Published:2010-12-29
Contact: CHEN Xue-lan E-mail:xuelanchen14@yahoo.com.cn

Abstract

Abstract:

Core-streptavidin gene Stv13 (354bp) has been cloned from Streptmyces avidinii using PCR amplification. Plasmids were constructed through inserting the Stv 13 gene into pET22b+ and pGEX-4T-1 vectors for expression in E. coli. Another plasmid was also constructed through inserting the Stv13 gene into P43 vector for expression in B. subtilis. Most of core-streptavidin gene Stv13 was expressed in inclusion body as an insoluble form in E. coli expression system. In contrast, little core-streptavidin gene Stv13 was expressed in B. subtilis expression system.

Key words: streptavidin, core-streptavidin, Escherichia coli, Bacillus subtilis

CLC Number:

R978.12
Q503

PENG Fu-zhong1，CHEN Xue-lan1,*，XU Feng2，XIONG Yong-hua2. Expression of Core-Streptavidin Gene in Prokaryotic Host[J]. FOOD SCIENCE, 2009, 30(23 ): 286-289.

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Expression of Core-Streptavidin Gene in Prokaryotic Host

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