FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (22 ): 101-103.doi: 10.7506/spkx1002-6300-200922020

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Use of Sephadex LH-20 Column Chromatography for Separation and Purification of Flavonoids in Artemisia selengensis Turcz

JIANG Hong,ZOU Zheng-rong   

  1. College of Life Science, Jiangxi Normal University, Nanchang 330022, China
  • Received:2009-07-21 Online:2009-11-15 Published:2010-12-29
  • Contact: ZOU Zheng-rong E-mail:zouzhr@163.com

Abstract:

The total flavonoids in Artemisia selengensis were extracted using ultrasonic-assisted organic solvent extraction method, purified with polyamide column chromatography, and further purified with Sephadex LH-20 column chromatography. Two flavonoid compounds, namely chrysoeriol-7-O-β-D-glucose and phloretin-3-O-β-D-xylose, were isolated, and identified according to their 1H and 13C-NMR data.

Key words: Sephadex LH-20 column chromatography, separation, purification

CLC Number: