Abstract: Diacylglycerol (DAG), a functional oil component, serves as a healthy substitute for triacylglycerols (TAG) oil. It enhances both the taste and nutritional value of foods. Among its isomers, 1,3-DAG offers specific health benefits: after metabolism in the human body, it reduces visceral fat accumulation, lowers blood lipids, and inhibits body mass gain. In recent years, it has attracted the attention of consumers. Comprehensive review of DAG preparation and purification technologies and its safety evaluation holds great importance for expanding its industrial applications. In this article, we first summarize the common preparation and purification technologies for DAG, highlighting their advantages, disadvantages and industrial application prospects. Next, the safety of DAG along with potential risk factors that merit attention is discussed. Furthermore, some examples of the application of DAG in various fields such as foods are presented. Finally, we explore future research directions and development trends. This review aims to provide a reference and technical support for the further development and utilization of DAG products.

Key words: diacylglycerol; preparation technology; purification technology; safety; applications