Purification of Saponins from Aralia continentalis Leaves Using Macroporous Adsorption Resin and Its Bioactivity

doi:10.7506/spkx1002-6630-200916031

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (16 ): 158-161.doi: 10.7506/spkx1002-6630-200916031

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Purification of Saponins from Aralia continentalis Leaves Using Macroporous Adsorption Resin and Its Bioactivity

FENG Ying1,2，LI Tian-lai2,*，MENG Xian-jun1，FAN Wen-li2

1. College of Food Science, Shenyang Agricultural University, Shenyang 110161, China；
2. College of Horticulture, Shenyang Agricultural University, Shenyang 110161, China

Received:2009-05-10 Online:2009-08-15 Published:2010-12-29

Abstract

Abstract:

Static absorption and desorption experiments were carried out to screen the optimum resin from 4 types of macroporous adsorption resins and macroporous resin HPD-100 was found to have the best performance for the purification of crude extract of saponins from Aralia continentalis leaves. In dynamic adsorption and desorption experiments, the optimum purification conditions of saponins by HPD-100 were determined as follows: saponin concentration 0.3 mg/ml, sampling volume 18 times of bed volume (BV), pH value of the sample 5, and gradient elution water → 10% ethanol → 30% ethanol → 50% ethanol. The eluates of 30% and 50% ethanol both had certain scavenging activity to superoxide radical and antibacterial activity.

Key words: Aralia continentalis, saponin, macroporous resins

CLC Number:

FENG Ying1,2，LI Tian-lai2,*，MENG Xian-jun1，FAN Wen-li2. Purification of Saponins from Aralia continentalis Leaves Using Macroporous Adsorption Resin and Its Bioactivity[J]. FOOD SCIENCE, 2009, 30(16 ): 158-161.

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Purification of Saponins from Aralia continentalis Leaves Using Macroporous Adsorption Resin and Its Bioactivity

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