Abstract:

Cottonseed protein was hydrolyzed by neutrase to produce active peptides. Single-factor method was adopted to investigate the effects of temperature, pH, enzyme amount and hydrolysis time on degree of hydrolysis (DH) of cottonseed protein. Subsequently, a central composite design (CCD) involving 17 experiments of three variables (i.e., temperature, pH and hydrolysis time) at three levels combined with response surface methodology was employed to attain the highest DH. When the amount of enzyme was 6000 U, the optimal temperature, pH value and hydrolysis time were 39.5 ℃, 7.5 and 5.5 h, respectively. Under the above conditions, the DH was 38.09%.

Key words: cottonseed protein, enzymatic hydrolysis, response surface methodology, optimization