Isolation, Identification and Antibacterial Activities of Endophytic Bacterial Strain XJ-EG-GA-13 from E.glauca Stems

doi:10.7506/spkx1002-6630-200913042

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (13 ): 179-182.doi: 10.7506/spkx1002-6630-200913042

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Isolation, Identification and Antibacterial Activities of Endophytic Bacterial Strain XJ-EG-GA-13 from E.glauca Stems

GULISHAN·Maimaiti，ZHAO Guo-yu，GOPUR·Mijit*

( College of Life Science and Technology, Xinjiang University, Urumqi 830046, China)

Received:2009-01-14 Online:2009-07-01 Published:2010-12-29
Contact: GOPUR·Mijit* E-mail:gmijit2001@yahoo.com.cn

Abstract

Abstract:

A novel endophytic bacterial strain XJ-EG-GB-13 against several species of plant pathogenic bacteria was isolated and identified from E.glauca stems by grinding method and agar diffusion test. The biological properties of the XJ-EG-GB-13 were also under this investigation. The results showed that the optimal growth temperature and pH value for the XJ-EG-GB- 13 were 37 ℃ and 6, respectively. According to morphological characteristics, physiological and biochemical properties and 16 S rDNA sequencing, the XJ-EG-GB-13 had 99.791% homology to Bacillus.subtilis subsp.subtilis. The results suggested that the XJ-EG-GB-13 is a potential and promising bacterium for studies of the mechanism of antagonist.

Key words: E.glauca, endophyte, antagonistic action, isolation, identification

CLC Number:

TS201.3

GULISHAN·Maimaiti，ZHAO Guo-yu，GOPUR·Mijit*. Isolation, Identification and Antibacterial Activities of Endophytic Bacterial Strain XJ-EG-GA-13 from E.glauca Stems[J]. FOOD SCIENCE, 2009, 30(13 ): 179-182.

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Isolation, Identification and Antibacterial Activities of Endophytic Bacterial Strain XJ-EG-GA-13 from E.glauca Stems

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