Abstract: To determine the fermented oral liquid production procedure of Agaricus blazei and to evaluate its immunomodulating activity. Methods: Concentrated submerged cultural broth of Agaricus blazei was blended with extract of the mecylium. And the Agaricus blazei oral liquid (AbOL) was prepared through the processes of fine filtration, preperation, bottling, sterilization, and cooling. Kunming mice were used to detect the effects of the oral liquid on immune activities (i.p.) with different polysaccharide dosages (5 mg/kg·d, 10 mg/kg·d and 30 mg/kg·d). The measured items included immune organ weight, chicken red blood cell phagocytic index of macrophage cell of abdominal cavity, hemoglobin amount analysis with chicken red blood cell as immune source, ELISA of lymphocyte in mice peripheral blood and DNFB-induced DTH. Results: Agaricus blazei oral liquid has the function to increase spleen and thymus index, phagocytic function of macrophage cell and hemoglobin amount. And it can enhance DNFB-induced delayed allergy of mice. The optimal dosage of polysaccharide is 10 mg/kg·d. Conclusion: Agaricus blazei oral liquid has the funtion of promoting unspecific immunity, specific immunity and cellular immunity of mice.

Key words: Agaricus blazei, healthy care oral liquid, polysaccharide, immunomodulating activity