FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (4): 272-277.

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Characteristics of Exoinulinase and Endoinulinase from Aspergillus ficuum

 WANG  Jing, JIN  Zheng-Yu, SUN  Bao-Guo, CAO  Yan-Ping   

  1. 1.College of Chemistry and Environment Engineering, Beijing Technology and Business University; 2.School of Food Science and Technology, Jiangnan University
  • Online:2008-04-15 Published:2011-08-24

Abstract: Characteristics of two types of inulin-degrading enzymes from Aspergillus ficuum were studied. The optimum reaction temperature of endo- and exo-inulinase were around 45 ℃. The optimum reaction pH of endo-inulinase was 5.0, and that of exo-inulinase was 4.5. Both of them were stable within the pH range 4.0~8.0 and below the temperature 50 ℃. The metal ions had the different level influences on the purified enzymes. Endo-and exo-inulinase activities were completely inhibited by Ag+, and obviously by Fe2+ and Al3+, while Mg2+ had an activating effect. In addition the kinetic constants of the two enzymes were also studied.

Key words: exoinulinase, endoinulinase, characteristic, Aspergillus ficuum