Abstract: A method has been developed for the simultaneous determination of the contents of galic acid, Catechin, (-)- epicatechin, Caffeic acid, pyrocatechol, Chlorogenic acid, flavone and Felinic acid in wines by high performance liquid chroma- tography- photodiode array detector (HPLC-PAD)with solid phase extraction (SPE). The techniques of SPE including the activation of the SPE column, elimination of interference and elution conditions of samples were confirmed. The analytes were separated by using a Hypersil Cl8 column (250 mm×4.6 mm, i.d. 5μm) and acetonitrile-0.3%HAc as mobile phase with linear gradient elution. The eight compounds were identified by retention time data and spectral characteristics and quantified by peak area. There were good linear correlations between the concentrations and peak areas of the eight components with the correlation coefficients of 0.9982～0.9999. The average recoveries were 83.9 %～103.7 % with relative standard deviations of 1.6 %～4.6 % (n=5). The detection limits were 0.2～1.5 mg/L. The method of SPE-HPLC-PAD eliminated a great deal of interferences in wines effectively by optimizing extraction conditions.The eight biological activity were well separated and the analytical results were improved well and truly. The results for determining 7 market wines showed that as an important bioactive substance. the phenolic compounds content has close relationships with brewing methods, varieties of grape and producing areas.The results also showed that it is a convenient, rapid, sensitive and accurate method. It can be used to analyze these biological activity in other complex matrices.

Key words: solid phase extraction, HPLC-PAD, wines, phenolic compounds