Abstract: A new method for the determination of organic acids in C.pinnatifida Bunge based on matrix solid phase dispersion was established. Organic acids were analyzed by high performance liquid chromatography with 0.01 mol/L NaH2PO4 buffer(pH 2.98) as mobile phase at a flow rate of 2.0 ml/min and detected at wavelength of 210 nm. The organic acids were separated on a ZORBAX Stable Bound (4.6 mm×50 mm, 1.8μm) column within 3 min. Limits of detections (S/N=3) were below 200μg/L. Mean recoveries ranged from 94% to 98%, and the relative standard deviations were below 3.0%. This method has been successfully applied to the determination of organic acids in C.pinnatifida Bunge with satisfactory results.

Key words: high performance liquid chromatography, organic acids, C.pinnatifida Bunge