Preparation and Evaluation of Lipid Nanoparticles for Loading Enzyme Subtilisin FS33

Abstract

Abstract: In order to increase bio-utilization and potentiate targeting of liposome, Subtilisin FS33 nano-liposome capsulation system was constructed. By single-factor and orthogonal design tests, the optimum preparation conditions obtained are as follows: Weight rate of cholesterol to lecithin 1:2, concentration of ammonium sulfate 0.15 mol/L, incubation temperature 45 ℃ and FS33 to lipid rate 1:1. Under the optimized conditions,the available encapsulation efficiency （EE） and maintenance efficiency （ME） of FS33 are 30.3% and 86.6%, respectively. The nano-liposome has a diameter of 50150 nm, which belongs to nano-single-room-liposome capsulation. Encapsulated FS33 in the liposome shows more tolerance to simulated intestinal juice than the original FS33 （p<0.01）.

Key words: fibrinolytic enzyme, ammonium sulfate gradient remote loading, nano-liposome, preparation, available encapsulation efficiency

WANG Cheng-Tao, JI Bao-Ping, CAO Yan-Ping, SUN Bao-Guo, HU Liang. Preparation and Evaluation of Lipid Nanoparticles for Loading Enzyme Subtilisin FS33[J]. FOOD SCIENCE, 2008, 29(9): 158-161.

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Preparation and Evaluation of Lipid Nanoparticles for Loading Enzyme Subtilisin FS33

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