FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (8): 445-447.

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Culture Technology of Kunyu Mountain Pholiota adiposa(Wild-type) in Liquid Medium

 WANG  Shu-Fang, BU  Qing-Mei, LIU  Lin-De, LIU  Jin-Jie, XU  Lu, LU Yan-Ling   

  1. 1.College of Life Science,Ludong University,Yantai 264025,China;2. Management Office of Laboratory and Equipment,Ludong University,Yantai 264025,China
  • Online:2008-08-15 Published:2011-08-26

Abstract: A suitable liquid medium for shaking culture of Kunyu Mountain Pholiota adipose(wild-type) was screened using single test statistics. Composition of the medium includs of 5.0 % corn meal,2.0 % wheat bran,0.1% KH2PO4,0.05 % MgSO·4 7 H2O and 100μg VB1/L;Under the conditions of pH 6.0~7.5,10 % inoculation,26 ℃ to 27 ℃,160 r/min and 150 ml medium in 500 ml flask,the recovered dried mycelium is 45 g/L.

Key words: wild Pholiota adiposa, liquid culture, single test factor