Study on Optimization of Extraction Technique of Glutathione from Beer Yeast Waster with Response Surface Methodlogy

Abstract

Abstract: With beer yeast waster as the raw material,the important effect factors such as among ratio of material to water,time,temperature and dosage of acetic acid were confirmed by the single factor tests primarily,then according to the Box-Benhnken center combined experimental design principles,the 3 most important effect factors were analyzed with RSM.The optimum conditions were obtained as follows:ratio of material and water 1:4,temperature 43.6 ℃,dosage of acetic acid 3.5%,and time 5.5 h.Under these conditions,the content of GSH red reach over 29%.

Key words: beer yeast waste, glutathione(GSH), extraction, response surface methodlogy(RSM)

SHAO Wei, LE Chao-Yin, TANG Ming, XIONG Ze. Study on Optimization of Extraction Technique of Glutathione from Beer Yeast Waster with Response Surface Methodlogy[J]. FOOD SCIENCE, 2008, 29(5): 173-176.

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Study on Optimization of Extraction Technique of Glutathione from Beer Yeast Waster with Response Surface Methodlogy

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