Abstract: Fungal diversity of wheat Qu of Shaoxing rice wine was studied by conventional dilution plate method and a culture-independent method RISA (ribosomal intergenic spacer analysis), combining with sequencing. Sixteen kinds of filamentous fungi were separated from wheat Qu and identified by internal transcribed spacer sequencing technology. Among all cultures, Absidia corymbifera, Rhizopus oryzae, Rhizomucor pusillus, Aspergillus oryzae and Aspergillus fumigatus were major fungis in wheat Qu. The results of RISA fingerprint analysis showed this culture-independent method could be used to study fungal diversity of wheat Qu. Different kinds of wheat Qu from three rice wine factories were compared by RISA fingerprint analysis method. The results showed the main fungi in wheat Qu from three districts were different obviously, and proved that regional environment factors influenced the wheat Qu fungal community significantly.

Key words: Shaoxing rice wine, wheat Qu, RISA, fungal diversity