Abstract: A highly sensitive, specific and credible method for the simultaneous determination of 20 sex hormones in muscle of swamp eel performed by GC-MS(Agilent 5975) was studied.Muscle tissues of swamp eel (5.0 g) were hydrolyzed by glucuronidase from Helix pomatia juice, extracted by tert-butyl methyl ether, defatted by iso-octane, and cleaned-up through dual columns of C18 and NH2. The extracts obtained were derivatized with heptafluorobutyric anhydride, following formation of the di-heptafluorobutyryl (di-HFB) derivative, and then identification and quantification were carried out by means of capillary gas chromatography-mass spectrometry. For quantitation, the external standards were used and the method was validated with blank samples fortified at 1, 2.5 and 5 μg/kg with 5.0 g sample(trenbolone spiked 5 times as others). Results showed that the detection limits are 0.25～5μg/kg for all analytes. Average recovery (%) and coefficient of variance range from79.8% to 106.3%, and from 11.6% to 17.9%, respectively. The relative standard deviation (RSD) ranges from 9.3% to 22.1% at 1 mg/kg spiked level. The study results showed that the recovery and repeatability are good. Therefore, the method is very useful for the animal food quality surveillance at present.

Key words: sex hormone, GC-MS, swamp eel