Study on Isolation and Physicochemical Characteristics of Water-soluble Polysaccharides from Gastrodia elata Blume

Abstract

Abstract: The experimental results showed that the optimum extraction parameters of water-soluble polysaccharides from Gastrodia elata Blume(WPGB) are 50 ℃, extraction time 3 h, ratio of solid to liquid 1:10 and ethanol concentration 80%. Under the conditions, the recovery of WPGB is about 9.67% (dry weight). WPGB-A-H and WPGB-A-L were separated from deproteined WPGB with DEAE-52 column and Sephadex G-100 column, successively. The recovery of WPGB-A-H is up to 0.824% (dry weight) and its purity is high by high-performance liquid chromatography. The identification results of qualitative chemical reaction showed WPGB-A-H is a neutral polysaccharide. It dose not contain proteins, nucleic acids, uronic acid and starch. The average molecular weight of WPGB-A-H is proved to be 28,840 D.

Key words: , Gastrodia elata Blume； polysaccharides； separation and purification； physicochemical properties；

MING Jian, GUI Ming-Ying, SUN Ya-Nan, CHEN Zong-Dao, ZHAO Guo-Hua. Study on Isolation and Physicochemical Characteristics of Water-soluble Polysaccharides from Gastrodia elata Blume[J]. FOOD SCIENCE, 2008, 29(9): 344-347.

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Study on Isolation and Physicochemical Characteristics of Water-soluble Polysaccharides from Gastrodia elata Blume

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