Response Surface Methodology for Optimization of Culture Conditions for the Production of Tetramethylpyrazine by Bacillus subtilis S0507

doi:10.7506/spkx1002-6630-201409043

Abstract

Abstract:

Tetramethylpyrazine (TTMP) was prepared from Bacillus subtilis S0507 under solid-state fermentation. Response
surface methodology (RSM) was applied to optimize the culture conditions. On the basis of the results of one-factor-at-atime
experiments, a Box-Behnken design (BBD) was used to investigate the interactive effect of culture temperature, time
and solid-to-liquid ratio (water content of the solid medium) on TTMP production, and the experimental data obtained
were analyzed with Design-Expert v8.0.1.6 software. The optimal culture conditions as determined by response
surface analysis were 33.84 h, 41.75 ℃ and water content of 59.63%. The measured yield of TTMP under the optimized
conditions was 332.70 mg/kg, showing a relative deviation of 0.84% in comparison with the theoretically predicted
value of 335.49 mg/kg. Thus, response surface methodology is feasible to optimize the culture conditions for TTMP
production by Bacillus subtilis S0507.

Key words: tetramethylpyrazine, Bacillus subtilis, response surface methodology, gas chromatography (GC)

ZHU Sai-feng, WU Jian-feng, ZHAO Xi-rong. Response Surface Methodology for Optimization of Culture Conditions for the Production of Tetramethylpyrazine by Bacillus subtilis S0507[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201409043.

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Response Surface Methodology for Optimization of Culture Conditions for the Production of Tetramethylpyrazine by Bacillus subtilis S0507

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