FOOD SCIENCE ›› 2006, Vol. 27 ›› Issue (3): 79-82.

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Properties and Substrate Specifity of Fructosyltransferase from Aspergillus sp.JN19

 WANG  Li-Mei, ZHOU  Hui-Ming   

  1. School of Food Science and Technology, Southern Yangtze University, Wuxi 214036, China
  • Online:2006-03-15 Published:2011-09-06

Abstract: The result indicated that the fructosyltransferase from a wild strain of Aspergillus sp.JN19 was a intracellular enzyme.The optimum temperature and pH for the high activity of fructosyltransferase were 50℃ and 5.5, respectively. Mg2+ ions enhancedfructosyltransferase activity by 2.2-fold whereas Cu2+ ions completely inhibited enzyme activity. The enzyme synthesized newfructosyl oligosacchrides by transfering fructose moiety of sucrose to various acceptors.

Key words: oligosaccharides, fructosyltransferse, substrate specifity