Study on Optimal Conditions of Assaying Pesticides Residue through Inhibition of Rabbit Serum Acetylcholinesterase

Abstract

Abstract: The conditions were studied for assaying of acetylcholinesterase (AChE) activity from rabbit serum, as the targetenzyme of organophosphate and carbamate pesticide. The results showed that the optimal reaction conditions were: 3.0ml of pH8.0buffer solution of phosphoric acid, 50μl of 0.6% DTNB and 100μl of 0.75% AChE were mixed with 50μl of 1% BTCI at 35℃,and then value detected of OD412 after 180s. The AChE activity showed good inhibition effect on the prothiofos, anorganophosphate pesticide, with 0.0125～0.4μg/ml as the concentration range and / or 5￣30 min as incubation time under the abovecondition. It indicated that the assay time could be adjusted. These results would be important to the R&D of rapid detectioncard for assaying pesticide residue by rabbit serum AChE and improveing test accuracy.

Key words: , pesticide residue； rapid detection； acetylcholinesterase； prothiofos；

WANG Wen-Xiang, HU Mei-Ying, LIU Xuan-Qing, PU Xiao-Ming, ZHONG Guo-Hua. Study on Optimal Conditions of Assaying Pesticides Residue through Inhibition of Rabbit Serum Acetylcholinesterase[J]. FOOD SCIENCE, 2006, 27(3): 179-183.

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Study on Optimal Conditions of Assaying Pesticides Residue through Inhibition of Rabbit Serum Acetylcholinesterase

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