DNA Extraction from Genetically Modified Foods by Modified CTAB Method or SDS Method

Abstract

Abstract: Object: To explore the DNA extraction methods for preparing DNA suitable for PCR-based detection of genetically modified organisms in foods and animals feed. Methods: DNAs of soybean, maize, tomato, potato and the processed products from these materials were extracted by using modified CTAB method or SDS method, and then were used as templates for PCR amplifying trnL gene of chloroplast as well as lectin gene of soybean and zein 10 gene of maize. Results: trnL gene of chloroplast and lectin gene of soybean and zein 10 gene of maize were revealed positive by PCR when using DNAs extraction by modified CTAB method as templates, while lectin and zein 10 were revealed negative by PCR amplifying of several DNA samples prepared by SDS method. Conclusion: DNA extract by using modified CTAB methods was suitable for PCR-based detection of genetically modified organisms in foods and animals feed.

Key words: genetically modified foods, DNA extraction, PCR

ZHOU Jian-Chang, YANG Ming-Jie, YANG Xing-Fen, HUANG Jun-Ming, LING Wen-Hua, HUANG Qiong, LI Wen-Li. DNA Extraction from Genetically Modified Foods by Modified CTAB Method or SDS Method[J]. FOOD SCIENCE, 2005, 26(2): 29-32.

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DNA Extraction from Genetically Modified Foods by Modified CTAB Method or SDS Method

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